Project information
Structure-function relationship of elicitors from Phytophthora spp.
- Project Identification
- GA522/06/0156
- Project Period
- 1/2006 - 12/2008
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Science
- Keywords
- plant protection, elicitor, Phytophthora, elicitin
Elicitins are a family of 10 kDa protein elicitors with high degree of homology excreted by all Phytophthora spp. that is the best-studied genus of Oomycetes.
One of the general accepted concepts admits that these elicitors are recognized by specific receptors and this primary interaction triggers defense reaction.Our previous results show that elicitins trigger two signal pathways in tobacco cells: the first one induces oxidative burst and the second one the expression of defense genes. We proposed and synthesized several mutated proteins with altered abilities to bind sterols and fatty acids and with altered abilities to stimulate these pathways. The aim this projet is the elucidation of the role and origine of the receptor. We will study individual signal components: cation a calcium channels, synthesis of phytoalexins, protecting activity of mutated proteins and changes of the proteom of the elicited tobacco cells. The results will enable the synthesis of the elicitors with directed properties. They will contribute to the developing new methods of plant protection without the use of pesticides.
Publications
Total number of publications: 9
2010
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Determination of capsidiol in tobacco cells culture by HPLC
Journal of Chromatographic Science, year: 2010, volume: 48, edition: 6
2009
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Interaction of cryptogein with its binding sites in tobacco plasma
Analytical biochemistry, year: 2009, volume: 390, edition: 2
2008
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Interakce cryptogeinu s vazebným místem na plazmatické membráně tabáku
Year: 2008, type: Conference abstract
2007
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Study of cryptogein recognition in plant defense reaction.
FEBS Journal, year: 2007
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Štúdium rannej fázy obrannej reakcie tabaku vyvolanej elicitínmi
Year: 2007, type: Conference abstract
2006
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Ergosterol is a signal molecule that leads to the expression of defence-related genes in tobacco
Year: 2006, type: Conference abstract
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Ergosterol treatment leads to the expression of a specific set of defence-related genes in tobacco
Plant Molecular Biology, year: 2006, volume: 62, edition: 1-2
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SYSTEMIC RESISTANCE OF PLANTS, THEORY VS. PRACTICE
Year: 2006, type: Conference abstract
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Výhody a úskalí produkce proteinů v expresním systému kvasinky Pichia pastoris
Year: 2006, type: Conference abstract